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Liver Extract Solid

No.

Instruction

 Initials
1 Treat the liver powder with acidified alcohol  
2 Cover & stir for 30 minutes  
3 Filter via vacuum and Buchner  
4 Treat solid residue with 50% ethyl alcohol  
5 Cover & stir for 30 minutes  
6 Filter via vacuum and Buchner  
7 Combine the two filtrates and Discard residue  
8 Filter the mixture to clarify (yield approximately 1000ml for 200Gm of liver powder)  
9 Evaporate the mixture in hood with heated air source (loose approximately 80% of the starting volume)  
10 Prepare a number of tarred filter papers (mark w pencil)  
11 Treat the concentrate with anhydrous or 95% ethyl alcohol.  Do in small amounts.  Swirl for 30 - 60 seconds.  Filter this carmel-colored suspension through weighed paper.  Continue using the same piece of paper until it seems fully loaded/clogged.  
12 Dry the filter papers  
13 The new filtrate may be concentrated again but each time the yield will be less.  It is probably not worth the effort to repeat the concentration/filtering process.  
14 Weigh the dried filter papers  
15 Subtract the weight of the blank filter papers from the weight of the filter paper that has material on it.  The difference is the amount of liver extract generated.  
16 Cut the filter paper into small pieces (use clean scissors)  
17 Place these small pieces in a beaker  
18 The final concentration is to be 20mg/ml (sterile).  So. use the weight determined above to calculate the final volume to be made.  Add 80% of this volume to the shards of paper.  
19 The active liver extract will dissolve off the paper  
20 Filter via vacuum and Buchner  
21 Filter through 0.8 micron filter and POSSIBLY a 0.45 micron  
22 Filter through 0.22 micron to sterilize  
23 Bring to final volume with SWFI  
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